Insights into metazoan evolution from Alvinella pompejana cDNAs.
Gagniere N, Jollivet D, Boutet I, Brelivet Y, Busso D, Da Silva C, Gaill F, Higuet D, Hourdez S, Knoops B, Lallier F, Leize-Wagner E, Mary J, Moras D, Perrodou E, Rees JF, Segurens B, Shillito B, Tanguy A, Thierry JC, Weissenbach J, Wincker P, Zal F, Poch O, Lecompte O.
BMC Genomics. 2010 Nov 16;11(1):634.
Alvinella pompejana, the "Pompeii worm", is a Polychaete Annelid (see taxonomy) considered as the most thermotolerant animal. This tubiculous worm colonizes hydrothermal vents where it is faced with extreme and variable physico-chemical conditions including very high temperatures (from 5 to 105 degrees Celsius), anoxic conditions, low ph, high concentration of heavy metals and sulphids... This environment makes A. pompejana an ideal model for studies aimed at deciphering adaptation in general as well as a unique source of thermostable proteins of eukaryotic origin.
On this web site, we present a searchable database of Alvinella pompejana sequences from four full length enriched cDNA libraries (whole animal, gills, pygidium and ventral tissue). Whole animals and dissected tissues have been collected during the oceanographic Biospeedo cruise on the Pacific Ridge. Full length cDNA libraries have been constructed and sequenced at the Genoscope (CNS). The database can be queried by blast or text search of annotations. Different views are available: nucleic cDNA sequence, EST trace and six-frame translation, contig schematic representation, Consed-like contig alignment view, MACSIMS annotated protein alignment with customisable features display, integrative view of the annotation (text mining definition, EC number, Gene Ontology, Pfam-A domains).
The sequences have been submitted to the EST section of the EMBL database under accession numbers FP489021 to FP539727 and FP539730 to FP565142.
This work was supported by institutional funds from INSERM, CNRS and UDS, by European Commission funding through the SPINE2-COMPLEXES project LSHG-CT-2006-031220 and by ANR-05-BLAN-0407 grant. The website is hosted by IGBMC.